Jaap Brink, Ph.D.

Electron crystallography and cryomicroscopy of macromolecular assemblies

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Who and where:

Jaap Brink, Ph.D.

W.M. Keck Center for Computational Biology and Verna and Marrs McLean Department of Biochemistry, Rm. 326B, Baylor College of Medicine, 1 Baylor Plaza, Houston TX 77030, U.S.A.

tel: +1 (713) 798-6989

fax: +1 (713) 796-9438

My geek code: Version: 3.1 GS d- s++: a+ C++++ LI++ P--- L+++ E W+++ N++ o+ K? w--- O! M V-->
PS+ PE Y-- PGP- t++@ 5 X- R- tv! b+ DI++ D@ G- e++++ h---- r++ y+++

For all your comments/suggestions on this and following pages, direct your e-mail to jbrink@bcm.tmc.edu

Research interests:

My research interest deals with the determination of the structure-function relationship of proteins using electron crystallography at the National Center for Macromolecular Imaging at the Baylor College of Medicine in Houston, Texas headed by Prof. Wah Chiu. The NCMI is an NIH supported resource center.

My work focuses currently on the following topics:

charging and its effects on high-resolution electron cryo-microscopy

3D reconstruction of the human Fatty Acid Synthase

Telemicroscopy

crystallization of a variety of proteins using the lipid monolayer technique

slow-scan CCD camera utilization for on-line assessment of specimen quality for data collection

Obtainable Software Packages
EDP -- GUI-based program for manual indexing and off-line processing of electron diffraction patterns. (archive \| tutorial)	AUTO -- Suite of FORTRAN 77-programs for completely automated indexing and processing of electron diffraction patterns. (archive \| tutorial)
PC2MRC -- Tool for converting SCANSALOT-format image files to MRC-format image files. (archive)	CONVGATAN -- Tool for converting Gatan-format image files to MRC-format image files. (archive)

Selected Papers:

Brink, J. and Chiu, W. (1994) Applications of a Slow-Scan CCD Camera in Protein Electron Crystallography. J. Struct. Biology, 113:23-34.

Brink, J. and Tam, M.W. (1996) Processing of electron diffraction patterns acquired on a slow-scan CCD camera. J. Struct. Biology, 116:144-150.

Sherman, M.B., Brink, J. and Chiu, W. (1996) Performance of a slow-scan CCD camera for macromolecular imaging in a 400 kV electron cryomicroscope. Micron, 27(2):129-139.

Brink, J., Sherman, M.B., Berriman, J. and Chiu, W. (1998) Evaluation of charging on macromolecules in electron cryomicroscopy. Ultramicroscopy, 72:41-52.

Brink, J., Gross, H., Tittmann, P., Sherman, M.B. and Chiu, W. (1998) Reduction of charging in protein electron cryomicroscopy. J. Microsc. In press.

Frey, W., Brink, J., Schief, Jr., W.R., Chiu, W. and Vogel, V. (1998) Projection structure of two-dimensional streptavidin crystals coordinated to metal-chelated lipid monolayers. Biophys. J., 74(5):2674-2679.

Brink. J., Ludtke, S.J., Yang, C.-Y., Gu, Z.-W., Wakil, S.J. and Chiu, W. (2002) Quaternary structure of human fatty acid synthase by electron cryomicroscopy. Proc. Natl. Acad. Sci. USA, 99(1):138-143.
All papers published so far are here.