Wensel Lab Protocols

Last Modified January, 2002

NOTE: All files are in PDF format.

I. General Protocols

• Westerns
• Northerns
• PCR
• RTPCR
• RACE
• PCR Mutagenesis
• Extinction Coeffecient Calculation
• Library Screening
• Immunocytochemistry

II. Protein Expression & Purification

• ROS
• Transducin from ROS
• Transducin-a from holo-transducin
• Transducin from E. coli
• PDE from ROS
• PDEg from E. coli
• GST-tagged proteins from E. coli
• His-tagged proteins from E. coli
• Baculovirus Expression System

III. Assays

• GAP Assay
• PDE pH Assay
• Phosphorylation
• Vesicle Binding
• RPA
• GDP-GTP Exchange Assay for transducin

IV. Equipment

• Waters HPLC
• Beckman HPLC
• Flourescence Microscope
• Electron Microscope

V. Computational Techniques

• Evolutionary Trace (see also Lichtarge Lab Homepage)
• Molecular Modeling using Swiss PDB Viewer
• Generating Molecular Graphics (images, animations)