Recent Publications from the Wensel Lab

Yao, C.-K., Lin, Y. Q, Ly, C. V. Ohyama, T., Haueter, C. M., Moiseenkova-Bell, V. Y., Wensel, T. G., Bellen, H. J. 2009. A synaptic vesicle- and presynaptic membrane-associated ion channel regulates resting Ca2+ levels and synaptic endocytosis. Cell, 138:947-60.

Commentary in Cell by Brose & Neher

Mancuso, J. J., Larson, A. M., Wensel, T. G., Saggau, P. 2009. Multiphoton Adaptation of a Commercial Low Cost Confocal Microscope for Live Tissue Imaging. J. Biomed. Opt. 14:034048.

Mojumder, D. K., Qian, Y., Wensel, T. G. 2009. Two R7 RGS proteins shape retinal bipolar cell signaling. J. Neurosci. 29:7753-7765.

Moiseenkova-Bell, V. Y., Wensel T. G. 2009. Hot on the trail of TRP channel structure. J Gen Physiol. 133:239-44

Sakamoto., K. McCluskey, M. Wensel, T.G., Naggert, J.K., Nishina, P.M. 2009. New Mouse Models for Recessive Retinitis Pigmentosa Caused by Mutations in the Pde6a Gene. Hum. Mol. Genet. 18:178-92.

Wang, Q., Zhang, X., Zhang, L., He, F., Zhang, G., Jamrich, M., Wensel, T.G. 2008. Activation-dependent hindrance of photoreceptor G protein diffusion by lipid microdomains. J. Biol. Chem., 283(44):30015-24.

Wensel, T.G. 2008. Signal Transducing Membrane Complexes of Photoreceptor Outer Segments. Vis. Res.48:2052-61.

Mojumder, D. K., Wensel, T. G., Frishman, L. J. 2008. Subcellular compartmentalization of two calcium binding proteins, calretinin and calbindin-28kDa, in ganglion and amacrine cells of the rat retina. Mol. Vis.14:1600-1613.

Gross, A.K., Wang, Q., Wensel, T.G. 2008 Regulation of Photoresponses by Phosphorylation. Chap. 6 in, Barnstable C.J., Tombran-Tink, J., eds., Visual Transduction and Non-Visual Light Perception, Humana Press, Towtowa NJ pp. 125-140.

Benfield, A., MacLeod, M., Liu, Y., Wu, Q., Wensel,T.G., Vasquez,K. 2008. Targeted generation of DNA strand breaks using pyrene-conjugated triplex-forming oligonucleotides" Biochemistry. 47:6279-88.

Moiseenkova-Bell, V. Y., Stanciu, L.A., Serysheva, I.I. Tobe, B.J. Wensel, T. G. 2008. Structure of TRPV1 channel revealed by electron cryomicroscopy. Proc. Natl. Acad. Sci. USA 105:7451-7455.

Jedema, H. P., Gold, S. J., Gonzalez-Burgos, G., Sved, A. F., Tobe, B. J., Wensel, T. G., Grace, A. A. 2008. Chronic cold exposure increases RGS7 expression and decreases α2-autoreceptor-mediated inhibition of noradrenergic locus coeruleus neurons. Eur. J. Neurosci. 27(9):2433-2443.

Abd-El-Barr, M.M., Albini, T.A., Carvounis, P.E., He, F., Manzano, R.P.A., Chevez-Barrios, P., Wensel, T.G., Wu, S.M, Holz, E.R, 2008 Safety and pharmokinetics of triamcinolone hexacetonide in rabbit eyes. J. Ocular Pharmacol. Ther. 24(2):197-205.

Wang, Q., Wensel, T.G. 2007 Biochemical characterization of phototransduction RGS9-1 GAP complex. Chap 5 in, Flieser, S. J., Kisselev, O., eds., Signal Transduction in the Retina. CRC Press, Boca Raton.

Morgans, C.W., Liu, W., Wensel, T.G., Bearnot, B., Perez, J., Brown, R.L., Duvoisin, R.M. (2007) Gbeta5-RGS complexes co-localize with mGluR6 in retinal ON-bipolar cells. Eur. J. Neurosci. 26:2899–2905.

Khajavi, M., Shiga, K., Wiszniewski, W., He, F. Shaw, C. A. Yan, J., Wensel, T. G., Snipes, G. J., Lupski, J. R. (2007) Oral curcumin mitigates the clinical and neuropathologic phenotype of the Trembler-J mouse: A potential therapy for inherited neuropathy. Am J. Hum Genet. 83:438-453.

Song, J.H., Song, H., Wensel TG, Sokolov, M., Martemyanov, K. A. (2007) Localization and differential interaction of R7 RGS proteins with their membrane anchors R7BP and R9AP in neurons of vertebrate retina. Mol Cell Neurosci.35:311-9.

Marc, R.E., Jones, B.W., Anderson, J.R., Kinnard, K., Marshak, D.W., Wilson, J.H., Wensel, T.G., Lucas, R.J. (2007) Neural reprogramming in retinal degenerations. Invest. Ophthalmol.Vis. Sci. 48:3364-71.

Zhang, X., Wensel, T. G., and Yuan, C. (2006). Tokay Gecko Photoreceptors Achieve Rod-like Physiology with Cone-like Proteins. Photochem Photobiol.82:1452.

Gross, A. K., Decker, G., Chan, F., Sandoval, I. M., Wilson, J. H., Wensel, T. G. (2006) Defective development of photoreceptor membranes in a mouse model of recessive retinal degeneration. Vision Res. 46:4510-4518.

Krispel, C. M. Chen, D., Chen, Y-J. Martemyanov, K. A., Quillinan, N., Arshavsky, V. Y., Wensel, T. G. Chen, C-K. Burns, M. E. (2006) RGS expression sets the duration of signaling in rod photoreceptors. Neuron 51:409-16.)

Commentary on Krispel et al. paper

Meltzer, R. H., Lurtz, M. M., Wensel, T. G., Pedersen, S.E. (2006) Nicotinic Acetylcholine Receptor Channel Electrostatics Determined by Diffusion Enhanced Luminescence Energy Transfer. Biophys. J. 91:1315-1324

Meltzer, R. H., Thompson, E., Soman, K., Song, X.-Z., Wensel, T. G.Briggs, J. M., Pedersen, S.E. (2006) Electrostatic Steering at Acetylcholine Binding Sites. Biophys. J. 91:1302-1314

Commentary on Meltzer AchR papers.

Iyer, M. N., He, F., Wensel, T. G., Mieler, W. F., Benz, M. S., Holz, E. R. (2006) Clearance of intravitreal moxifloxacin. Invest. Ophthalmol. Vis. Sci. 47:317-319.

Wensel, T. G., Gross, A. K., Chan, F., Sykoudis, K., Wilson, J. H. (2005) Rhodopsin-EGFP knock-ins for imaging quantal gene alterations. Vis. Res. 45:3445-3453.

Wiszniewski, W., Zaremba, C. M., Yatsenko, A. M., Jamrich, M., Wensel, T., Lupski, J. R., Lewis, R. A. (2005) ABCA4 mutations causing mislocalization are found frequently in patients with severe retinal dystrophies. Hum. Mol. Genet. 14:2769-2778 COVER

Wensel, T.G., He, F., Malinski, J.A. 2005. Purification, reconstitution on lipid vesicles, and assays of PDE6 and its activator G protein, transducin. Methods Mol. Biol. 307:289-314.

Hu, G., Wensel, T. G. 2004. Characterization of R9AP, a membrane anchor for the photoreceptor GAP, RGS9-1. Methods Enzymol. 390:178-196.

Zhang, Z., Melia, T. J., He, F., Yuan, C., McGough, A., Schmid, M. F., Wensel, T.G., 2004. How a G-protein binds a membrane. J. Biol. Chem. 279:33937-33945

Chan, F., Bradley, A., Wensel, T.G., Wilson, J.H. 2004. Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy . Proc. Natl. Acad. Sci. USA 101:9109-9114

He, F., Mao, M., Wensel, T.G. 2004. Enhancement of phototransduction G protein-effector interactions by phosphoinositides J. Biol. Chem. 279:8986-90.

Madabushi, S., Gross, A. K., Philippi, A., Meng, E. C., Wensel, T. G., Lichtarge, O. 2004. Evolutionary trace of G protein-coupled receptors reveals clusters of residues that determine global and class-specific functions. J. Biol. Chem. 279:8126-32.

Wilson, J.H., Wensel, T.G. 2003. The nature of dominant mutations of rhodopsin and implications for gene therapy. Mol. Neurobiol. 28:149–158.

Zhang, L., El-Hodiri, H. M., Ma, H. -F. Zhang, X., Servetnick, M., Wensel, T. G., and Milan Jamrich, M. 2003. Targeted expression of the dominant negative FGFR-4a in the eye using Xrx1A regulatory sequences interferes with normal retinal development. Development, 130:4177-4186.

Burns, M.E., Wensel, T.G. 2003. From molecules to behavior: new clues for RGS function in the striatum. Neuron 38:853–856.

Chen, C. -K., Eversole-Cire, P., Zhang, H., Macino, V., Chen, Y. -J., He, W., Wensel, T. G., Simon, M. I. 2003. Instability of GGL domain-containing RGS proteins in mice lacking the G-protein beta subunit Gbeta5. Proc. Natl. Acad. Sci. USA 100:6604-6609.

Hu, G, Zhang, Z., Wensel, T. G. 2003. Activation of RGS9-1 GTPase acceleration by its membrane anchor, R9AP. J. Biol. Chem. 278:14550-14554.

Sokal, I., Hu, G., Liang, Y., Mao, M., Wensel, T.G., Palczewski, K. 2003. Identification of protein kinase C isozymes responsible for the phosphorylation of photoreceptor-specific RGS9-1 at SER475. J. Biol. Chem. 275:8316-8325.

Kennedy,M.J., Sowa, M.E., Wensel, T. G., Hurley, J.B. 2003. Acceleration of key reactions as a strategy to elucidate the rate limiting chemistry underlying phototransduction inactivation. Invest. Ophthal. Vis. Sci. 44:1016-1022.

Hu, G. and Wensel, T. G. 2002. R9AP, an anchor protein for the photoreceptor GAP RGS9-1. Proc. Natl. Acad. Sci. USA 99:9755-9760.

Wensel, T.G. 2002. RGS9-1 phosphorylation and calcium. Chapter 8 in W. Baehr and K. Palczewski, eds., Photoreceptors and Calcium. eurekah.com, Landes Bioscience, Georgetown, TX pp. 125-129

Sierra, D.A., Gilbert, D.J., Householder, D. Grishin, N.V., Yu, K., Ukidwe, P., Barker, S., He, W., Wensel, T.G., Otero, G., Brown, G., Copeland, N.G., Jenkins, N.A., Wilkie, T.M. 2002. Evolution of The Regulators of G Protein Signaling (RGS) Multigene Family in Mouse and Human,  Genomics 79:177-185.

Imanishi, Y., Li, N., Sokal, I., Sowa, M. E., Lichtarge, O., Wensel, T. G., Saperstein, D. A., Baehr, W., Palczewski, K. 2002. Characterization of Retinal Guanylate cyclase-Activating protein 3 (GCAP3) From Zebrafish to man. Eur. J. Neurosci. 65.15:63-78.

He, W. and Wensel, T. G. 2002. Approaches to RGS function in visual signal transduction. Methods Enzymol. 344:725-740.

He., W., Cowan, C.W., Melia, T.J., Jr., Wensel, T.G. 2001. Dependence of RGS9-1 membrane attahment on its C-terminal tail. J. Biol. Chem.  276:48961-48966.

Hu, G., Jang, G.-F., Cowan, C. W., Wensel, T. G., Palczewski, K. 2001. Phosphorylation of RGS9-1 by an endogenous protein kinase in bovine rod outer segments. J. Biol. Chem., 276: 22287-22295.

Shroyer, N. F., Lewis, R. A., Yatsenko, A., Wensel, T. G., Lupski, J. R. 2001. Cosegregation and functional analysis of mutant ABCR (ABCA4) alleles in families that manifest both Stargardt disease and age-related macular degeneration. Human Mol. Genet., 10:2671-2678

Lyubarsky, A. L., Chen, C. -K., Naarendorp, F., Zhang, X., Wensel, T., Simon, M. I., Pugh, E. N., Jr. 2001. RGS9-1 is required for normal inactivation of mouse cone phototransduction. Mol. Vis. 7:71-78.

Sowa, M.E., He, W., Slep, K. C., Kercher, M. A., Lichtarge, O., Wensel, T. G. 2001. Prediction and Confirmation of a Site Critical for Effector Regulation of RGS Domain Activity. Nature Structural Biology, 8:234-237   (COVER)

Slep, K. C., Kercher, M. A., He, W., Cowan, C. W., Wensel, T. G., Sigler, P. B. 2001. Structural determinants for regulation of phosphodiesterase by a G-protein at 2.0 Å. Nature, 409:1071-1077.

Cowan, C. W., He, W., Wensel, T. G. 2001. RGS Proteins: Lessons from the RGS9 subfamily. Progress in Nucleic Acid Research and Molecular Biology 65:341-359.

He, W., Lu, L, Zhang, X., El-Hodiri, H. M., Chen, C.-K., Slep, K.C., Simon, M.I., Jamrich, M., and Wensel, T. G., 2000. Modules in the photoreceptor RGS9-1/Gb5L GAP complex control effector coupling, GTPase acceleration, protein folding, and stability. J. Biol. Chem., 275:37093-37100.

Intody, Z., Perkins, B. D., Wilson, J. H, Wensel, T. G., 2000. Blocking transcription of the Human Rhodopsin Gene by Triplex-Mediated DNA Photocrosslinking. Nucleic Acids Res. Nucleic Acids Res. 28:4283-4290.

He, F., Seryshev, A., Cowan, C.W., Wensel, T. G. 2000. Multiple zinc binding sites in retinal rod cGMP phosphodiesterase, PDE6alpha beta. J. Biol. Chem. .275: 20572-20577.

Womack, K. B., Gordon, S. E., He, F., Wensel, T. G., Lu, C.-C., Hilgemann, D. W. 2000. Do phosphatidylinositides modulate vertebrate photoransduction ? J. Neurosci. 20:2792-2799.

Sowa, M. E., He, W., Wensel, T. G. and Lichtarge, O. 2000. A regulator of G protein signaling interaction surface linked to effector specificity. Proc. Natl. Acad. Sci. USA 97:1483-1488.

Chen, C.-K., Burns, M. E., He, W., Wensel, T. G., Baylor, D. A., Simon, M. I. 2000. Slowed recovery of rod photoresponse in mice lacking the GTPase accelerating protein RGS9-1. Nature. 403:557-560.

Melia, T. J., Jr., Malinski, J. A., He, F. and Wensel, T. G. 2000. Enhancement of phototransduction protein interactions by membrane surfaces. J. Biol. Chem. 275:3535-3542.

Kovoor, A., Chen, C. -K., He, W., Wensel, T. G., Simon, M. I., Lester, H. A. 2000. Co-expression of Gb5 enhances the function of two Gg subunit-like domain-containing regulators of G protein signaling proteins. J. Biol. Chem. 275:3397-3402.

Cowan, C. W., Wensel, T.G., Arshavsky, V.Y. 2000. Enzymology of GTPase acceleration in vision. Methods Enzymol., K. Palczewski, ed. 315:524-538.

Melia, T.J., Sowa, M.E., Schutze, L., Wensel, T. G. 1999. Formation of helical protein assemblies of IgG and transducin on varied lipid tubules.  J. Struct. Biol. 128:119-130.

Perkins, B. D., Wensel, T. G., Vasquez, K. M., Wilson, J. H. 1999. Psoralen Photo-Crosslinking by Triplex-Forming Oligonucleotides at Multiple Sites in the Human Rhodopsin Gene. Biochemistry 38:12850-12859.

Zhang, K., Howes, K., He, W., Pettenati, M., Palczewski, K., Wensel, T. G., Baehr, W. 1999. Structure, alternative splicing, and expression of human photoreceptor GTPase regulatory protein (RGS9) variants. Gene 240:23-34.

Rahman, Z., Gold, S. J., Potenza, M. N., Cowan, C. W., Ni, Y. G., He, W., Wensel, T. G., Nestler , E. J., 1999. Cloning and characterization of RGS9-2: a striatal-enriched alternatively spliced product of the RGS9 gene. J. Neurosci. 19:2016-2026.

DeMar, J. C., Rundle, D. R., Wensel, T. G., Anderson, R. E. 1999. Heterogeneous N-terminal acylation of retinal proteins. Progress in Lipid Research, 38:49-90.

Wensel, T. G. 1999 Heterotrimeric G Proteins: Structure, regulation, and signaling mechanisms. Chapter 2 in A. Sitaramayya, ed., Introductory Signal Transduction, Birkhauser, Boston, pp. 29-46.

Perkins, B. D., Wilson, J. H., Wensel, T. G., Vasquez, K. M. 1998.  Triplex targets in the human rhodopsin gene.  Biochemistry.  37:11315-11322.

Cowan, C.W., Fariss, R. N., Palczewski, K., Wensel, T. G. 1998 High expression levels in cones of RGS9, the predominant GTPase accelerating protein of rods.  Proc. Natl. Acad. Sci. USA 95:5351-5356.

He, W., Cowan, C. W., Wensel, T. G. 1998  RGS9, a GTPase accelerator for phototransduction.  Neuron 20:95-102.

Melia, T. J. Jr., Cowan, C. W., Angleson, J. K. and Wensel, T. J. 1997 A comparison of the efficiency of G protein activation by ligand-free and light-activated forms of rhodopsin. Biophysical Journal 73:3182-3191.

DeMar, J. D., Wensel, T. G. and Anderson, R. E. 1996. Biosynthesis of the unsaturated 14-carbon fatty acids found on the N-termini of photoreceptor specific proteins.  J. Biol. Chem. 271: 5007- 5016.

Malinski, J. A.,  Zera, E.Z., Angleson, J. K. and Wensel, T.G. 1996 High affinity interactions of GTPgammaS with the heterotrimeric G protein, transducin: Evidence at low and high protein concentrations.   J. Biol. Chem. 271: 12919-12924.

Zera, E. Z., Molloy, D. P., Angleson, J. K., Lamture, J.B., Wensel, T.G., and Malinski, J. A. 1996 Low affinity interactions of GDPbetaS and ribose- or phosophoryl-substituted GTP analogues with heterotrimeric G protein, transducin.  J. Biol. Chem. 271: 12925-12931.

Vasquez, K. M., Wensel, T. G., Hogan, M. E. and Wilson, J. H. 1996 High efficiency triple helix- mediated photocrosslinking at a targeted site within a selectable mammalian gene.  Biochemistry, 35:10712-10719.

Lamture, J. B., Zhou, Z., Suresh Kumar, A. , and Wensel, T. G. 1995. Luminescence properties of Tb(III) complexes with 4-substituted dipicolinic acid analogues. Inorganic Chemistry, 34:864-869.

Lamture, J. B. and Wensel, T. G. 1995. Intensely Luminescent Immunoreactive Conjugates Of Proteins And Dipicolinate-Based Polymeric Tb(III) Chelates.  Bioconjugate Chemistry, 6:88-92.

Vasquez, K. M., Wensel, T. G., Hogan, M. E., and Wilson, J. H. 1995.  High affinity triple helix formation by synthetic oligonucleotides at a site within a selectable mammalian gene. Biochemistry 34:7243-7251.

Wensel, T. G. and  Angleson, J. K. 1995. More answers about cGMP-gated channels pose more questions.  Behavioral and Brain Sciences, 18:492-493.

Angleson, J.K., and Wensel, T.G. 1994. Enhancement of rod outer segment GTPase accelerating protein activity by the inhibitory subunit of cGMP phosphodiesterase.   J. Biol. Chem. 269:16290-16296.

Lamture, J. B. and Wensel, T. G.  1993.  A novel reagent for labelling macromolecules with intensely luminescent lanthanide complexes.  Tetrahedron Lett. 34: 4141-4144.

Wensel, T.G.  1993.  The light-regulated cGMP phosphodiesterase of vertebrate photoreceptors: Structure and mechanism of activation by Gta. Chap. 56  in Dickey, B. and Birnbaumer, L., eds., GTPases in Biology II, Springer-Verlag, Berlin, Heidelberg, New York. pp 213-223

Angleson, J.K., and Wensel, T. G. 1993. A GTPase accelerating factor for transducin, distinct from its effector phosphodiesterase, in rod outer segment membranes.  Neuron, 11:939-949.

LAST UPDATED 07-15-2009