Prediction and confirmation of a site critical for effector regulation of RGS domain activity
Mathew E. Sowa, Wei He, Kevin C. Slep, Michele A. Kercher, Olivier Lichtarge & Theodore G. Wensel
pp 234 - 237

Residues that regulate the activity of the RGS family (regulators of G protein signaling) were pre-dicted using the evolutionary trace method, which combines sequence and structure data to infer the location of functional sites in proteins, and the results were confirmed using site-directed mutagenesis. These residues (green balls) are mapped onto the structure of RGS9 (gray) in complex with G (cyan) and phosphodiesterase (purple). See pages 234-237; the structure of the complex is presented in a separate paper (Slep et al. Nature, 2001, 409:1071-1077).