Education
B.S. Chemistry, Centenary College (1998)
Ph.D. SCBMB, Baylor College of Medicine (2007)
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Mike Marsh
Baylor College of Medicine
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Department: Postdoctoral Fellow, Biochemistry & Molecular Biology
Address: One Baylor Plaza, Room N420
Houston, TX 77030
Phone: 713-798-6989
Fax: 713-798-8031
Email: michael.marsh@bcm.tmc.edu
Web: mikemarsh.googlepages.com/home
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Honors
| 1996 | Outstanding Achievement | Lousiana Summer Scholars Program. Biochemistry, LSU Medical Center | | 1998 | Best Seminar, Natural Sciences | Centenary College Research Forum | | 1998 - 1999 | Rockwell Scholar | Graduate School of Biomedical Sciences, Baylor College of Medicine | | 1999 - 2003 | Predoctoral Fellow | W.M. Keck Center for Computational Biology | tr> 2003 - 2004 | Predoctoral Fellow | Welsh Foundation | | 2000 | Best Poster | SCBMB Annual Retreat |
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Research Topic
Structural Analysis of Human Adenovirus by Electron Cryomicroscopy
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Research Description
Adenovirus is a human pathogen known to infect and replicate in the eye, respiratory tract, gastrointestinal tract, and urinary bladder. It is likely the causative agent responsible for 3% of all infections in the general population, including 10% of childhood pneumonias. Because of its ability to infect a large variety of non-dividing cells, there has been a recent surge in interest in adenovirus as a gene-delivery vector for use in clinical gene therapy. The single greatest impediment to long-lasting expression by adenoviral vectors has been host immune response. We have recently started work on determining the intermediate resolution (7 - 10 Å) structure of the human adenovirus virion by electron cryomicroscopy. A structural analysis at this resolution should lead to a more complete understanding of the features of the capsid proteins and their structural organization. In addition to the whole capsid study, we are working on a structural analaysis of the adenovirus fiber protein. Study of this protein should aid our understanding of how adenovirus is able to specifically target certain cell types, and how that targeting may be modified. This may prove helpful in the intelligent design of adenoviral vectors that can 1) evade the hosts' immune response and 2) target new cell types, thereby broadening their applicability in gene therapy.
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Selected Publications
- Madabushi S, Yao H, Marsh M, Kristensen D, Philippi A, Sowa ME, Lichtarge O (2002) Structural Clusters of Evolutionary Trace Residues are Statistically Significant and Common in Proteins, J Mol Biol, 316:139-154
- McKevitt M, Patel K, Smajs D, Marsh M, Norris SJ, Weinstock GM, Palzkill T (2003) Cloning and Expression of the Treponema pallidum Proteome, Genome Res, 13: 1665-74
- Campos SK, Parrott MB, Marsh M, Chiu W, Barry MA (2004) Metabolically biotinylated viruses for vector targeting, virus purification, and capsid imaging, Mol. Ther. 9: S390.
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